pGMV-U
(Plasmid
#112797)
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Purpose(Empty Backbone) Universal BeYDV-derived vector for cloning of up to four gRNAs
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112797 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTC223
- Backbone size (bp) 15615
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Vector typePlant Expression, CRISPR
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Selectable markersNone
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Addgene NGS results identified an IS5 element immediately upstream of KanR, which should not affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGMV-U was a gift from Humberto Prieto (Addgene plasmid # 112797 ; http://n2t.net/addgene:112797 ; RRID:Addgene_112797) -
For your References section:
Grape Biotechnology: Past, Present, and Future. Prieto H, Miccono M, Aguirre C, Sánchez E, Castro A. In: Cantu D., Walker M. (eds) The Grape Genome. Compendium of Plant Genomes. Springer, Cham. 10.1007/978-3-030-18601-2_16.
Map uploaded by the depositor.
