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Addgene

pGMV-gSWEET4
(Plasmid #112796)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112796 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGMV-U
  • Backbone manufacturer
    n/a
  • Total vector size (bp) 14985
  • Vector type
    Plant Expression, CRISPR
  • Selectable markers
    None

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA pair targeting VviSWEET4
  • gRNA/shRNA sequence
    VviSWEET4
  • Species
    Vitis vinifera

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer TCAAAAGTCCCACATCGCTTAGA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS results identified an IS1 element immediately upstream of KanR, which should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGMV-gSWEET4 was a gift from Humberto Prieto (Addgene plasmid # 112796 ; http://n2t.net/addgene:112796 ; RRID:Addgene_112796)

  • For your References section:

    Grape Biotechnology: Past, Present, and Future. Prieto H, Miccono M, Aguirre C, Sánchez E, Castro A. In: Cantu D., Walker M. (eds) The Grape Genome. Compendium of Plant Genomes. Springer, Cham. 10.1007/978-3-030-18601-2_16.
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