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His6-Twin-Strep-SUMO-pET28a-EfaCas2
(Plasmid #112794)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112794 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    His6-Twin-Strep-SUMO-pET28a
  • Backbone size w/o insert (bp) 5800
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    EfaCas2
  • Species
    Synthetic
  • Tag / Fusion Protein
    • His6-Twin-Strep-SUMO (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site cgcGGATCCATGAGTTATCGATATATGCGTC (destroyed during cloning)
  • 3′ cloning site CCGCTCGAGTTATTCTTCTCCTAAAAAGACA (destroyed during cloning)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His6-Twin-Strep-SUMO-pET28a-EfaCas2 was a gift from Ailong Ke (Addgene plasmid # 112794 ; http://n2t.net/addgene:112794 ; RRID:Addgene_112794)

  • For your References section:

    How type II CRISPR-Cas establish immunity through Cas1-Cas2-mediated spacer integration. Xiao Y, Ng S, Nam KH, Ke A. Nature. 2017 Oct 5;550(7674):137-141. doi: 10.1038/nature24020. Epub 2017 Sep 4. 10.1038/nature24020 PubMed 28869593
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