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Addgene

pBP-PliaG
(Plasmid #112777)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112777 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSB1C3
  • Backbone manufacturer
    iGEM
  • Backbone size w/o insert (bp) 2115
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PliaG promoter
  • Alt name
    BBa_K143012
  • Species
    Synthetic
  • Insert Size (bp)
    121

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bsa1 (unknown if destroyed)
  • 3′ cloning site Bsa1 (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2017/09/06/185108 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBP-PliaG was a gift from Chris French (Addgene plasmid # 112777 ; http://n2t.net/addgene:112777 ; RRID:Addgene_112777)

  • For your References section:

    BacilloFlex: A modular DNA assembly toolkit for Bacillus subtilis synthetic biology. Wicke N, Radford D, Verrone V, Wipat A, French CE. bioRxiv 185108 10.1101/185108
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