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pLJM1 Flag GFP 4E-BP1 Rheb15 CAAX
(Plasmid #112762)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112762 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLVX
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Flag-GFP-4EBP1-Rheb15 (CAAX mutant)
  • Species
    H. sapiens (human)
  • Mutation
    CAAX mutation to prevent lipid localization
  • Entrez Gene
    EIF4EBP1 (a.k.a. 4E-BP1, 4EBP1, BP-1, PHAS-I)
  • Tag / Fusion Protein
    • Rheb15(CAAX mutant) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLJM1 Flag GFP 4E-BP1 Rheb15 CAAX was a gift from Roberto Zoncu (Addgene plasmid # 112762 ; http://n2t.net/addgene:112762 ; RRID:Addgene_112762)

  • For your References section:

    A nutrient-induced affinity switch controls mTORC1 activation by its Rag GTPase-Ragulator lysosomal scaffold. Lawrence RE, Cho KF, Rappold R, Thrun A, Tofaute M, Kim DJ, Moldavski O, Hurley JH, Zoncu R. Nat Cell Biol. 2018 Sep;20(9):1052-1063. doi: 10.1038/s41556-018-0148-6. Epub 2018 Jul 30. 10.1038/s41556-018-0148-6 PubMed 30061680
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