pET-2(KW)2-mCherry
(Plasmid #112738)

• Purpose: Express 2(KW)2-mCherry in E. coli
• Depositing Lab: Kyubong Jo
• Publication: Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6. doi: 10.1093/nar/gkv834. Epub 2015 Aug 11.

Backbone

• Vector backbone: pET-15b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5706
• Total vector size (bp): 6456
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 2(KW)2-mCherry
• Species: E. coli
• Insert Size (bp): 753
• Promoter: T7 Promoter
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7 Promoter
• 3′ sequencing primer: T7 Terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: The mCherry insert contains a N9Q mutation. The depositor has confirmed this does not affect plasmid function.