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pET-2(KW)2-eGFP
(Plasmid #112736)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112736 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-15b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5706
  • Total vector size (bp) 6489
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    2(KW)2-eGFP
  • Species
    E. coli
  • Insert Size (bp)
    783
  • Promoter T7 Promoter
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 Promoter
  • 3′ sequencing primer T7 Terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-2(KW)2-eGFP was a gift from Kyubong Jo (Addgene plasmid # 112736 ; http://n2t.net/addgene:112736 ; RRID:Addgene_112736)

  • For your References section:

    DNA binding fluorescent proteins for the direct visualization of large DNA molecules. Lee S, Oh Y, Lee J, Choe S, Lim S, Lee HS, Jo K, Schwartz DC. Nucleic Acids Res. 2016 Jan 8;44(1):e6. doi: 10.1093/nar/gkv834. Epub 2015 Aug 11. 10.1093/nar/gkv834 PubMed 26264666
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