pAAV hSyn GFP-Fxr1
(Plasmid #112732)

• Purpose: AAV plasmid that expresses GFP-Fxr1 fusion protein under hSYN promoter
• Depositing Lab: Martin Beaulieu
• Publication: Khlghatyan et al Front Mol Neurosci. 2018 Apr 12;11:119. doi: 10.3389/fnmol.2018.00119. eCollection 2018.

Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 4547
• Total vector size (bp): 7199
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Fxr1p
• Species: M. musculus (mouse)
• Insert Size (bp): 1863
• Entrez Gene: Fxr1 (a.k.a. 1110050J02Rik, 9530073J07Rik, Fxr1h, Fxr1p)
• Promoter: hSYN
• Tag / Fusion Protein:
  • GFP (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GCGGAGCTGACGGTGGAG
• 3′ sequencing primer: TTATGAAACACCATTCAGGACTGCTG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry