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pcDNA3-FLAG-RNMT 121-476
(Plasmid #112707)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112707 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5446
  • Total vector size (bp) 6567
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RNMT
  • Alt name
    RNA guanine-7 methyltransferase
  • Alt name
    Cap methyltransferase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1131
  • Mutation
    deleted amino acids 2-120
  • Entrez Gene
    RNMT (a.k.a. CMT1, CMT1c, MET, Met, N7-MTase, RG7MT1, cm1p, hCMT1, hMet)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (destroyed during cloning)
  • 3′ cloning site KpnI (destroyed during cloning)
  • 5′ sequencing primer T7 promoter (TAATACGACTCACTATAGG)
  • 3′ sequencing primer SP6 promoter (ATTTAGGTGACACTATAG)
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Source of RNMT sequence: pET16b-His-RNMT from Stuart Shuman, Sloan Kettering

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-FLAG-RNMT 121-476 was a gift from Daniel Schoenberg (Addgene plasmid # 112707 ; http://n2t.net/addgene:112707 ; RRID:Addgene_112707)

  • For your References section:

    RNA guanine-7 methyltransferase catalyzes the methylation of cytoplasmically recapped RNAs. Trotman JB, Giltmier AJ, Mukherjee C, Schoenberg DR. Nucleic Acids Res. 2017 Oct 13;45(18):10726-10739. doi: 10.1093/nar/gkx801. 10.1093/nar/gkx801 PubMed 28981715
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