pCMV-NFIX2ERT2
(Plasmid
#112704)
-
PurposeExpression of NFIX2-ERT2 fusion protein
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 112704 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCMV
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNuclear Factor I X2
-
Alt nameNfix2
-
SpeciesM. musculus (mouse)
-
Entrez GeneNfix (a.k.a. CTF, NF-I/X, NF1-X, NFI-X)
-
Tags
/ Fusion Proteins
- HA (N terminal on insert)
- ERT2 (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made byThe Cre materials originated from a Jackson Labs material (https://www.jax.org/strain/008463).
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCMV-NFIX2ERT2 was a gift from Richard Gronostajski (Addgene plasmid # 112704 ; http://n2t.net/addgene:112704 ; RRID:Addgene_112704)