pCAGG-NFIX1
(Plasmid
#112702)
-
PurposeExpression of NFIX1 protein and GFP
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 112702 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCAGG
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNuclear Factor I X1
-
Alt nameNfix1
-
SpeciesM. musculus (mouse)
-
Entrez GeneNfix (a.k.a. CTF, NF-I/X, NF1-X, NFI-X)
-
Tags
/ Fusion Proteins
- HA (N terminal on insert)
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made byThe GFP in this plasmid was obtained from the Guillemot lab that is now part of the Crick Institute.
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCAGG-NFIX1 was a gift from Richard Gronostajski (Addgene plasmid # 112702 ; http://n2t.net/addgene:112702 ; RRID:Addgene_112702)