pGEX-2T-NM
(Plasmid
#1127)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1127 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-2T
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Backbone manufacturerpharmacia
- Backbone size w/o insert (bp) 4952
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNM
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Alt nameSup35
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Alt nameSup35 NM region
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)761
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GenBank IDAY553988
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Entrez GeneSUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
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Tags
/ Fusion Proteins
- GST
- GST
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pGEX5'
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-2T-NM was a gift from Susan Lindquist (Addgene plasmid # 1127 ; http://n2t.net/addgene:1127 ; RRID:Addgene_1127) -
For your References section:
The role of conformational flexibility in prion propagation and maintenance for Sup35p. Scheibel T, Lindquist SL. Nat Struct Biol 2001 Nov;8(11):958-62. 10.1038/nsb1101-958 PubMed 11685242