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Addgene

pCAGG-NFIX2
(Plasmid #112699)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112699 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nuclear Factor I X2
  • Alt name
    Nfix2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Nfix (a.k.a. CTF, NF-I/X, NF1-X, NFI-X)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The GFP in this plasmid was obtained from the Guillemot lab that is now part of the Crick Institute.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGG-NFIX2 was a gift from Richard Gronostajski & Francois Guillemot (Addgene plasmid # 112699 ; http://n2t.net/addgene:112699 ; RRID:Addgene_112699)

  • For your References section:

    Epigenomic enhancer annotation reveals a key role for NFIX in neural stem cell quiescence. Martynoga B, Mateo JL, Zhou B, Andersen J, Achimastou A, Urban N, van den Berg D, Georgopoulou D, Hadjur S, Wittbrodt J, Ettwiller L, Piper M, Gronostajski RM, Guillemot F. Genes Dev. 2013 Aug 15;27(16):1769-86. doi: 10.1101/gad.216804.113. 10.1101/gad.216804.113 PubMed 23964093
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