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Addgene

pCMV-NFIA1.1
(Plasmid #112698)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112698 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nuclear Factor I A1.1
  • Alt name
    Nfia1.1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Nfia (a.k.a. 1110047K16Rik, 9430022M17Rik, CTF, NF1-A, NF1A)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-NFIA1.1 was a gift from Richard Gronostajski (Addgene plasmid # 112698 ; http://n2t.net/addgene:112698 ; RRID:Addgene_112698)
  • For your References section:

    Nuclear factor I-mediated repression of the mouse mammary tumor virus promoter is abrogated by the coactivators p300/CBP and SRC-1. Chaudhry AZ, Vitullo AD, Gronostajski RM. J Biol Chem. 1999 Mar 12;274(11):7072-81. PubMed 10066764