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Addgene

gRNAs[bTub+Tra].1046D
(Plasmid #112693)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112693 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    white+attB
  • Vector type
    CRISPR
  • Selectable markers
    mini-white

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    U6.3-gRNA[bTub] & U6.3-gRNA[Tra]
  • gRNA/shRNA sequence
    CCTGAGTGTGCATCAGCTGG and CGGCGAGAAAGAGAATACCA
  • Species
    D. melanogaster (fly)
  • Entrez Gene
    tra (a.k.a. Dmel_CG16724, CG16724, Dmel\CG16724, Dmtra, TRA, Tra)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    gRNAs[bTub+Tra].1046D was a gift from Omar Akbari (Addgene plasmid # 112693 ; http://n2t.net/addgene:112693 ; RRID:Addgene_112693)

  • For your References section:

    Transforming insect population control with precision guided sterile males with demonstration in flies. Kandul NP, Liu J, Sanchez C HM, Wu SL, Marshall JM, Akbari OS. Nat Commun. 2019 Jan 8;10(1):84. doi: 10.1038/s41467-018-07964-7. 10.1038/s41467-018-07964-7 PubMed 30622266
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