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Addgene

nos-Cas9.874Z1
(Plasmid #112685)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112685 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    piggyBac+attB
  • Vector type
    Insect Expression
  • Selectable markers
    dsRed

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hSpCas9
  • Species
    Streptococcus pyogenes
  • Mutation
    Humanized Cas9 bacterial sequence
  • Promoter nanos
  • Tags / Fusion Proteins
    • NLS (N terminal on backbone)
    • 3x FLAG (N terminal on insert)
    • EGFP (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    nos-Cas9.874Z1 was a gift from Omar Akbari (Addgene plasmid # 112685 ; http://n2t.net/addgene:112685 ; RRID:Addgene_112685)

  • For your References section:

    Transforming insect population control with precision guided sterile males with demonstration in flies. Kandul NP, Liu J, Sanchez C HM, Wu SL, Marshall JM, Akbari OS. Nat Commun. 2019 Jan 8;10(1):84. doi: 10.1038/s41467-018-07964-7. 10.1038/s41467-018-07964-7 PubMed 30622266
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