pOTTC1442 - pAAV CMV-IE Nuc-iRFP-2A-iCre
(Plasmid
#112683)
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PurposeAn AAV vector that expresses a nuclear-localized iRFP713 reporter and improved Cre recombinase
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112683 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepOTTC1031
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Backbone manufacturerNIDA OTTC
- Backbone size w/o insert (bp) 4500
- Total vector size (bp) 6857
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameiRFP713
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SpeciesRhodopseudomonas palustris
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GenBank IDJN247409
- Promoter CMV-IE
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Tags
/ Fusion Proteins
- Nuclear localization signal (N terminal on insert)
- 2A-iCre (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CMVf
- 3′ sequencing primer WPRE_R1 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byiRFP was obtained from Addgene 31857 iCre was obtained from R. Sprengel, DOI 10.1002/gene.10023.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pOTTC1442 - pAAV CMV-IE Nuc-iRFP-2A-iCre was a gift from Brandon Harvey (Addgene plasmid # 112683 ; http://n2t.net/addgene:112683 ; RRID:Addgene_112683) -
For your References section:
Neuron-Specific Genome Modification in the Adult Rat Brain Using CRISPR-Cas9 Transgenic Rats. Back S, Necarsulmer J, Whitaker LR, Coke LM, Koivula P, Heathward EJ, Fortuno LV, Zhang Y, Yeh CG, Baldwin HA, Spencer MD, Mejias-Aponte CA, Pickel J, Hoffman AF, Spivak CE, Lupica CR, Underhill SM, Amara SG, Domanskyi A, Anttila JE, Airavaara M, Hope BT, Hamra FK, Richie CT, Harvey BK. Neuron. 2019 Feb 8. pii: S0896-6273(19)30062-5. doi: 10.1016/j.neuron.2019.01.035. 10.1016/j.neuron.2019.01.035 PubMed 30792150
Map uploaded by the depositor.
