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Addgene

pQE30-mBFP
(Plasmid #112682)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112682 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE30
  • Backbone manufacturer
    Qiagen
  • Backbone size w/o insert (bp) 3461
  • Total vector size (bp) 4174
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Growth instructions
    This mBFP is highly expressed as functional form without adding IPTG.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    His-mBFP
  • Alt name
    metagenomic derived blue-fluorescent protein
  • Species
    metagenome-derived gene
  • Insert Size (bp)
    786
  • GenBank ID
    GU460154.1
  • Promoter T5
  • Tag / Fusion Protein
    • His tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SphI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pQE-F
  • 3′ sequencing primer pQE-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE30-mBFP was a gift from Geun-Joong Kim (Addgene plasmid # 112682 ; http://n2t.net/addgene:112682 ; RRID:Addgene_112682)

  • For your References section:

    Screening of a highly soluble and oxygen-independent blue fluorescent protein from metagenome. Hwang CS, Choi ES, Han SS, Kim GJ. Biochem Biophys Res Commun. 2012 Mar 23;419(4):676-81. doi: 10.1016/j.bbrc.2012.02.075. Epub 2012 Feb 20. 10.1016/j.bbrc.2012.02.075 PubMed 22382031
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