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Addgene

pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2
(Plasmid #112668)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112668 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZ P CAGGS tdTPH (AddGene plasmid 112668)
  • Backbone size w/o insert (bp) 8900
  • Total vector size (bp) 10275
  • Vector type
    Mammalian Expression, AAV ; Donor plasmid for recombinase-mediated cassette exchange
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tdT
  • Alt name
    tandem dimer Tomato
  • Insert Size (bp)
    1428

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZ P TetON-3XFLAG-tdT CAGG-m2rtTA v2 was a gift from Catherine Verfaillie (Addgene plasmid # 112668 ; http://n2t.net/addgene:112668 ; RRID:Addgene_112668)

  • For your References section:

    Efficient Recombinase-Mediated Cassette Exchange in hPSCs to Study the Hepatocyte Lineage Reveals AAVS1 Locus-Mediated Transgene Inhibition. Ordovas L, Boon R, Pistoni M, Chen Y, Wolfs E, Guo W, Sambathkumar R, Bobis-Wozowicz S, Helsen N, Vanhove J, Berckmans P, Cai Q, Vanuytsel K, Eggermont K, Vanslembrouck V, Schmidt BZ, Raitano S, Van Den Bosch L, Nahmias Y, Cathomen T, Struys T, Verfaillie CM. Stem Cell Reports. 2015 Nov 10;5(5):918-931. doi: 10.1016/j.stemcr.2015.09.004. Epub 2015 Oct 8. 10.1016/j.stemcr.2015.09.004 PubMed 26455413
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