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Addgene

pZ:F3-CAGGS GPHTK-F
(Plasmid #112666)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112666 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pZ donor AAVS1 vector (CompoZr Targeted Integration Kit, Sigma Aldrich)
  • Backbone size w/o insert (bp) 4570
  • Total vector size (bp) 4566
  • Vector type
    AAV ; Geme targeting vector for integration at the S1 site
  • Selectable markers
    Hygromycin ; Thymidine kinase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    eGFP
  • Alt name
    GFP
  • Insert Size (bp)
    717

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pZ:F3-CAGGS GPHTK-F was a gift from Catherine Verfaillie (Addgene plasmid # 112666 ; http://n2t.net/addgene:112666 ; RRID:Addgene_112666)

  • For your References section:

    Efficient Recombinase-Mediated Cassette Exchange in hPSCs to Study the Hepatocyte Lineage Reveals AAVS1 Locus-Mediated Transgene Inhibition. Ordovas L, Boon R, Pistoni M, Chen Y, Wolfs E, Guo W, Sambathkumar R, Bobis-Wozowicz S, Helsen N, Vanhove J, Berckmans P, Cai Q, Vanuytsel K, Eggermont K, Vanslembrouck V, Schmidt BZ, Raitano S, Van Den Bosch L, Nahmias Y, Cathomen T, Struys T, Verfaillie CM. Stem Cell Reports. 2015 Nov 10;5(5):918-931. doi: 10.1016/j.stemcr.2015.09.004. Epub 2015 Oct 8. 10.1016/j.stemcr.2015.09.004 PubMed 26455413
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