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p415 GFP-Glo3 Δ2x+ve
(Plasmid #112662)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112662 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    P415 Met25
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP- Glo3 Δ2x+ve
  • Alt name
    YER122C
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2241
  • Mutation
    Mutations (R223E, K224E, K225E, K233E, K234E, K235E) affecting COPI binding
  • Promoter Met25
  • Tag / Fusion Protein
    • sfGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba1 (not destroyed)
  • 3′ cloning site Xma1 (not destroyed)
  • 5′ sequencing primer GGCACCTTGTCCAATTGAACACGCTCGATG
  • 3′ sequencing primer TTTTCAGTATAATGTTACATGCGTACACGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p415 GFP-Glo3 Δ2x+ve was a gift from Blanche Schwappach (Addgene plasmid # 112662 ; http://n2t.net/addgene:112662 ; RRID:Addgene_112662)

  • For your References section:

    Dissection of GTPase-activating proteins reveals functional asymmetry in the COPI coat of budding yeast. Arakel EC, Huranova M, Estrada AF, Rau EM, Spang A, Schwappach B. J Cell Sci. 2019 Aug 29;132(16). pii: jcs.232124. doi: 10.1242/jcs.232124. 10.1242/jcs.232124 PubMed 31331965
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