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Addgene

pCFD5_w
(Plasmid #112645)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112645 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCFD6
  • Backbone manufacturer
    Fillip Port and Simon Bullock
  • Backbone size w/o insert (bp) 6700
  • Total vector size (bp) 7791
  • Vector type
    Insect Expression
  • Selectable markers
    mini-white

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    U6:3-tRNA-sgRNA expression cassette
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1000

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid by Fillip Port and Michael Boutros.

A detailed cloning protocol is available at http://crisprflydesign.org/grna-expression-vectors/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCFD5_w was a gift from Michael Boutros (Addgene plasmid # 112645 ; http://n2t.net/addgene:112645 ; RRID:Addgene_112645)
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