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PurposeUbiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 112645 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCFD6
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Backbone manufacturerFillip Port and Simon Bullock
- Backbone size w/o insert (bp) 6700
- Total vector size (bp) 7791
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Vector typeInsect Expression
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Selectable markersmini-white
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameU6:3-tRNA-sgRNA expression cassette
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SpeciesD. melanogaster (fly)
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Insert Size (bp)1000
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid by Fillip Port and Michael Boutros.
A detailed cloning protocol is available at http://crisprflydesign.org/grna-expression-vectors/
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCFD5_w was a gift from Michael Boutros (Addgene plasmid # 112645 ; http://n2t.net/addgene:112645 ; RRID:Addgene_112645)
