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Addgene

pCR-Blunt_hNANOG
(Plasmid #112639)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112639 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR-Blunt
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 3500
  • Total vector size (bp) 4550
  • Vector type
    PCR Template

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hNANOG
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1086
  • Entrez Gene
    NANOG
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer M13 Fw
  • 3′ sequencing primer M13 Rv
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR-Blunt_hNANOG was a gift from Ganna Bilousova (Addgene plasmid # 112639 ; http://n2t.net/addgene:112639 ; RRID:Addgene_112639)

  • For your References section:

    RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells. McGrath PS, Diette N, Kogut I, Bilousova G. J Vis Exp. 2018 Nov 26;(141). doi: 10.3791/58687. 10.3791/58687 PubMed 30531717
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