pQE80L TriCatcher-RGDSP-MMP
(Plasmid #112633)

• Purpose: Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site, an MMP-cleavable sequence and a C-terminal SnoopCatcher
• Depositing Lab: Mark Howarth
• Publication: Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi: 10.1016/j.biomaterials.2018.07.020. Epub 2018 Jul 17.

Backbone

• Vector backbone: pQE80L
• Backbone manufacturer: Qiagen Inc.
• Backbone size w/o insert (bp): 4714
• Total vector size (bp): 6556
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TriCatcher-RGDSP-MMP
• Species: Synthetic
• Insert Size (bp): 1842
• Mutation: central matrix metalloproteinase-cleavable sequence and RGDSP integrin-binding sequence
• Promoter: T5
• Tags / Fusion Proteins:
  • His6 (N terminal on insert)
  • TEV cleavage site (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CATAAAAAATTTATTTGCTTTGTGAGCGG
• 3′ sequencing primer: CGAGCGTTCTGAACAAATCC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry