TCRalpha-L1
(Plasmid
#11262)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11262 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTripIEx2
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3600
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTCR alpha
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Alt nameTCRalpha-L1
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1315
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GenBank IDAF424545
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Entrez Genetrac (a.k.a. TCR-alpha, TCR1, TCR8RC, TCRA3, TCRAC, si:busm1-172d23.25, si:busm1-172d23.26, si:busm1-172d23.27, si:dz172d23.25, si:dz172d23.26, si:dz172d23.27)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To make probes: antisense EcoRI - T7, sense XhoI - T3.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TCRalpha-L1 was a gift from Lisa Steiner (Addgene plasmid # 11262 ; http://n2t.net/addgene:11262 ; RRID:Addgene_11262) -
For your References section:
T cells and the thymus in developing zebrafish. Danilova N, Hohman VS, Sacher F, Ota T, Willett CE, Steiner LA. Dev Comp Immunol. 2004 Jun . 28(7-8):755-67. 10.1016/j.dci.2003.12.003 PubMed 15043944