Ikaros
(Plasmid
#11261)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11261 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescriptII SK+
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameikaros
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)2300
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GenBank IDAF092175
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Entrez Geneikzf1 (a.k.a. ikaros, znfn1a1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To make probes: antisense SalI - T3, sense XbaI - T7.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ikaros was a gift from Lisa Steiner (Addgene plasmid # 11261 ; http://n2t.net/addgene:11261 ; RRID:Addgene_11261) -
For your References section:
Ikaros expression as a marker for lymphoid progenitors during zebrafish development. Willett CE, Kawasaki H, Amemiya CT, Lin S, Steiner LA. Dev Dyn. 2001 Dec . 222(4):694-8. 10.1002/dvdy.1223 PubMed 11748838