foxn4
(Plasmid
#11260)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 11260 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript KS-
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namefoxn4
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)1297
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Entrez Genefoxn4 (a.k.a. whn, whna)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To make probes: antisense XbaI - T3, sense XhoI - T7.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
foxn4 was a gift from Lisa Steiner (Addgene plasmid # 11260 ; http://n2t.net/addgene:11260 ; RRID:Addgene_11260) -
For your References section:
Expression of the winged helix/forkhead gene, foxn4, during zebrafish development. Danilova N, Visel A, Willett CE, Steiner LA. Brain Res Dev Brain Res. 2004 Oct 15. 153(1):115-9. 10.1016/j.devbrainres.2004.05.014 PubMed 15464224