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Addgene

pJC25NM
(Plasmid #1126)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 1126 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJC25
  • Backbone size w/o insert (bp) 2326
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NM stop
  • Alt name
    Sup35
  • Alt name
    Sup35 NM region
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    761
  • GenBank ID
    ay553988
  • Entrez Gene
    SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJC25NM was a gift from Susan Lindquist (Addgene plasmid # 1126 ; http://n2t.net/addgene:1126 ; RRID:Addgene_1126)

  • For your References section:

    The role of conformational flexibility in prion propagation and maintenance for Sup35p. Scheibel T, Lindquist SL. Nat Struct Biol 2001 Nov;8(11):958-62. 10.1038/nsb1101-958 PubMed 11685242
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