pGAT
(Plasmid #112587)

• Purpose: (Empty Backbone) Used for expression of protein in E. coli as Thrombin cleavable 6His-tag-GST fusions with a blunt end restriction site immediately after thrombin recognition sequence
• Depositing Labs: Marko Hyvönen, Johan Peränen
• Publication: Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Backbone

• Vector backbone: pBAT4
• Vector type: Bacterial Expression
• Tag / Fusion Protein:
  • 6His-GST (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • 6His-GST (N terminal on backbone)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry