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Addgene

Lentiviral-S-tdTomato
(Plasmid #112579)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112579 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti-C-Myc-DDK
  • Backbone manufacturer
    Origene
  • Backbone size w/o insert (bp) 6400
  • Total vector size (bp) 7800
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NLS-tdTomato-NES
  • Alt name
    Shuttle-tdTomato, S-tdTomato
  • Species
    Fluorescent protein tdTomato
  • Insert Size (bp)
    1300
  • Tags / Fusion Proteins
    • nuclear localization sequence (N terminal on insert)
    • nuclear export sequence (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    From Svetlana Vidensky, a technician in the Rothstein lab.
  • Articles Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lentiviral-S-tdTomato was a gift from Jeffrey Rothstein (Addgene plasmid # 112579 ; http://n2t.net/addgene:112579 ; RRID:Addgene_112579)

  • For your References section:

    The C9orf72 repeat expansion disrupts nucleocytoplasmic transport. Zhang K, Donnelly CJ, Haeusler AR, Grima JC, Machamer JB, Steinwald P, Daley EL, Miller SJ, Cunningham KM, Vidensky S, Gupta S, Thomas MA, Hong I, Chiu SL, Huganir RL, Ostrow LW, Matunis MJ, Wang J, Sattler R, Lloyd TE, Rothstein JD. Nature. 2015 Sep 3;525(7567):56-61. doi: 10.1038/nature14973. Epub 2015 Aug 26. 10.1038/nature14973 PubMed 26308891
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