pExp-TFSumo
(Plasmid #112576)

• Purpose: (Empty Backbone) Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and TF (Trigger factor (tig) from E. coli linked to Sumo) fusions with optional C-terminal StrepII-tag
• Depositing Lab: Marko Hyvönen
• Publication: Protein expressionists toolkit (unpublished)

Backbone

• Vector backbone: pOP2S
• Vector type: Bacterial Expression
• Tags / Fusion Proteins:
  • 8xHis-TFSumo (N terminal on backbone)
  • Strep tag (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tags / Fusion Proteins:
  • 8xHis-TFSumo (N terminal on backbone)
  • Strep tag (C terminal on backbone)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains N-terminal 8xHis, fusion partner: TF-Sumo (Trigger factor from E. coli (UniProtKB: P0A850) linked to Sumo (Smt3 from Saccharomyces cerevisiae, UniProtKB: Q12306), PMID: 26297996) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII