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Addgene

pExp-GB1
(Plasmid #112565)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112565 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pOP2S
  • Vector type
    Bacterial Expression
  • Tags / Fusion Proteins
    • 8xHis-GB1 (N terminal on backbone)
    • Strep tag (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tags / Fusion Proteins
    • 8xHis-GB1 (N terminal on backbone)
    • Strep tag (C terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Contains N-terminal 8xHis, fusion partner: GB1 (domain B1 of Immunoglobulin G-binding protein G from Streptococcus sp., UniProtKB: P19909, PMID: 9385638) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pExp-GB1 was a gift from Marko Hyvönen (Addgene plasmid # 112565 ; http://n2t.net/addgene:112565 ; RRID:Addgene_112565)