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Purpose(Empty Backbone) Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 fusions with optional C-terminal StrepII-tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 112565 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepOP2S
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Vector typeBacterial Expression
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Tags
/ Fusion Proteins
- 8xHis-GB1 (N terminal on backbone)
- Strep tag (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- 8xHis-GB1 (N terminal on backbone)
- Strep tag (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Contains N-terminal 8xHis, fusion partner: GB1 (domain B1 of Immunoglobulin G-binding protein G from Streptococcus sp., UniProtKB: P19909, PMID: 9385638) followed by TEV protease cleavage site, optional C-terminal StrepII-tag. Cloning of GOI between BsaI and XhoI or HindIII
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pExp-GB1 was a gift from Marko Hyvönen (Addgene plasmid # 112565 ; http://n2t.net/addgene:112565 ; RRID:Addgene_112565)