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pmiR-report-hVEGFA
(Plasmid #112553)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112553 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmiR-report
  • Backbone manufacturer
    Ambion
  • Vector type
    Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VEGFA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1644
  • Entrez Gene
    VEGFA (a.k.a. L-VEGF, MVCD1, VEGF, VPF)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (unknown if destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

clone into pmiR-report using SacI/HindIII

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmiR-report-hVEGFA was a gift from Shusheng Wang (Addgene plasmid # 112553 ; http://n2t.net/addgene:112553 ; RRID:Addgene_112553)

  • For your References section:

    Strand and Cell Type-specific Function of microRNA-126 in Angiogenesis. Zhou Q, Anderson C, Hanus J, Zhao F, Ma J, Yoshimura A, Wang S. Mol Ther. 2016 Oct;24(10):1823-1835. doi: 10.1038/mt.2016.108. Epub 2016 May 19. 10.1038/mt.2016.108 PubMed 27203443
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