pmiR-report-hARPC2
(Plasmid
#112549)
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Purpose3'UTR reporter assay
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 112549 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmiR-report
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Backbone manufacturerAmbion
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameARPC2
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SpeciesH. sapiens (human)
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Insert Size (bp)335
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Entrez GeneARPC2 (a.k.a. ARC34, PNAS-139, PRO2446, p34-Arc)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
clone into pmiR-report using SacI/HindIII
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmiR-report-hARPC2 was a gift from Shusheng Wang (Addgene plasmid # 112549 ; http://n2t.net/addgene:112549 ; RRID:Addgene_112549) -
For your References section:
Regulation of intraocular pressure by microRNA cluster miR-143/145. Li X, Zhao F, Xin M, Li G, Luna C, Li G, Zhou Q, He Y, Yu B, Olson E, Gonzalez P, Wang S. Sci Rep. 2017 Apr 19;7(1):915. doi: 10.1038/s41598-017-01003-z. 10.1038/s41598-017-01003-z PubMed 28424493