Skip to main content
Addgene

Adeno-miR-27b
(Plasmid #112539)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112539 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAdEasy
  • Backbone manufacturer
    Agilent
  • Vector type
    Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    miR-27b
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    291
  • Entrez Gene
    MIR27B (a.k.a. MIR-27b, MIRN27B, miRNA27B)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

clone into pShuttle-CMV using KpnI/NotI first. There exists a deletion of "AGC" at positions 285-287 compared to the depositor sequence that the depositing lab confirms is not of concern for function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Adeno-miR-27b was a gift from Shusheng Wang (Addgene plasmid # 112539 ; http://n2t.net/addgene:112539 ; RRID:Addgene_112539)

  • For your References section:

    Regulation of angiogenesis and choroidal neovascularization by members of microRNA-23~27~24 clusters. Zhou Q, Gallagher R, Ufret-Vincenty R, Li X, Olson EN, Wang S. Proc Natl Acad Sci U S A. 2011 May 17;108(20):8287-92. doi: 10.1073/pnas.1105254108. Epub 2011 May 2. 10.1073/pnas.1105254108 PubMed 21536891
Related items: