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Addgene

Adeno-miR-23b
(Plasmid #112538)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112538 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pShuttle
  • Backbone manufacturer
    Agilent
  • Vector type
    Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    miR-23b
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    274
  • Entrez Gene
    MIR23B (a.k.a. MIRN23B, hsa-mir-23b, miRNA23B, mir-23b)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer pF1ori-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

clone into pShuttle-CMV using KpnI/NotI first

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Adeno-miR-23b was a gift from Shusheng Wang (Addgene plasmid # 112538 ; http://n2t.net/addgene:112538 ; RRID:Addgene_112538)

  • For your References section:

    Regulation of angiogenesis and choroidal neovascularization by members of microRNA-23~27~24 clusters. Zhou Q, Gallagher R, Ufret-Vincenty R, Li X, Olson EN, Wang S. Proc Natl Acad Sci U S A. 2011 May 17;108(20):8287-92. doi: 10.1073/pnas.1105254108. Epub 2011 May 2. 10.1073/pnas.1105254108 PubMed 21536891
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