pMM1162
(Plasmid #112535)

• Purpose: pZE2-hrtR-RBS3-chuA - LuxCDABE transcriptional unit deletion of plasmid pMM643, ColE1 origin, KanR
• Depositing Lab: Timothy Lu
• Publication: Mimee et al Science. 2018 May 25;360(6391):915-918. doi: 10.1126/science.aas9315.

Backbone

• Vector backbone: PZE2
• Backbone size w/o insert (bp): 2216
• Total vector size (bp): 4976
• Modifications to backbone: RBS3 (stronger HrtR RBS)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: HrtR
• Species: Lactococcus lactis
• Insert Size (bp): 570
• Promoter: ProD

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTC AAT ACG AGG ACG CAG AAA GGG TG

Gene/Insert 2

• Gene/Insert name: ChuA
• Species: E. coli
• Insert Size (bp): 1983
• Promoter: J23107

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CCG TTC GTA AGC CAT TTC CGC TC

Resource Information

• Supplemental Documents:
  • pMM1162.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene NGS results detected a 23 bp deletion upstream of the ChuA ORF. The depositing lab indicates that this should not affect plasmid function.