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Addgene

pMM534
(Plasmid #112530)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112530 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PZE2
  • Backbone size w/o insert (bp) 2216
  • Total vector size (bp) 13951
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    luxCDABE
  • Alt name
    Bacterial luciferase operon
  • Species
    Photorhabdus luminescens
  • Insert Size (bp)
    5798
  • Promoter PL(HrtO)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains tandem copies of the luciferase operon which are both functional.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMM534 was a gift from Timothy Lu (Addgene plasmid # 112530 ; http://n2t.net/addgene:112530 ; RRID:Addgene_112530)

  • For your References section:

    An ingestible bacterial-electronic system to monitor gastrointestinal health. Mimee M, Nadeau P, Hayward A, Carim S, Flanagan S, Jerger L, Collins J, McDonnell S, Swartwout R, Citorik RJ, Bulovic V, Langer R, Traverso G, Chandrakasan AP, Lu TK. Science. 2018 May 25;360(6391):915-918. doi: 10.1126/science.aas9315. 10.1126/science.aas9315 PubMed 29798884
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