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Addgene

pSopBC460S
(Plasmid #112514)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112514 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWSK29
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sopB (catalytically inactive)/sigE operon
  • Alt name
    sigDE (catalytically inactive)
  • Species
    Salmonella enterica serovar Typhimurium
  • Mutation
    SopB C460S
  • Promoter PsopB

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSopBC460S was a gift from Olivia Steele-Mortimer (Addgene plasmid # 112514 ; http://n2t.net/addgene:112514 ; RRID:Addgene_112514)

  • For your References section:

    A second wave of Salmonella T3SS1 activity prolongs the lifespan of infected epithelial cells. Finn CE, Chong A, Cooper KG, Starr T, Steele-Mortimer O. PLoS Pathog. 2017 Apr 20;13(4):e1006354. doi: 10.1371/journal.ppat.1006354. eCollection 2017 Apr. 10.1371/journal.ppat.1006354 PubMed 28426838
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