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Addgene

pSopB2xHA
(Plasmid #112513)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 112513 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pWSK29
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sopB-2xHA/sigE operon
  • Alt name
    sigDE-2HA
  • Species
    Salmonella enterica serovar Typhimurium
  • Mutation
    SopB C-terminal 2xHA
  • Promoter PsopB
  • Tag / Fusion Protein
    • 2xHA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSopB2xHA was a gift from Olivia Steele-Mortimer (Addgene plasmid # 112513 ; http://n2t.net/addgene:112513 ; RRID:Addgene_112513)

  • For your References section:

    Ubiquitination of the bacterial inositol phosphatase, SopB, regulates its biological activity at the plasma membrane. Knodler LA, Winfree S, Drecktrah D, Ireland R, Steele-Mortimer O. Cell Microbiol. 2009 Nov;11(11):1652-70. doi: 10.1111/j.1462-5822.2009.01356.x. Epub 2009 Jul 13. 10.1111/j.1462-5822.2009.01356.x PubMed 19614667
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