pIGAhRC
(Plasmid #112510)

• Purpose: human Ah receptor and Arnt cDNAs expressed under control of Gal1,10 bidirectional promoter
• Depositing Lab: Charles Miller
• Publication: Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3):297-303. doi: 10.1006/taap.1999.8769.

Backbone

• Vector backbone: unknown
• Vector type: Yeast Expression, Synthetic Biology
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: HIF1B, AhR
• Species: H. sapiens (human), S. cerevisiae (budding yeast)
• Entrez Gene: ARNT (a.k.a. ARNT1, HIF-1-beta, HIF-1beta, HIF1-beta, HIF1B, HIF1BETA, TANGO, bHLHe2)
• Entrez Gene: AHR (a.k.a. RP85, bHLHe76)
• Promoter: Gal1,10

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: GAL1

Resource Information

• Supplemental Documents:
  • pIGAHRC.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains the human aryl hydrocarbon receptor (AhR) expressed from the GAL1,10 promoter along with ARNT. Together they form a heterodimeric transcription factor.

Use the HpaI site for linearization and integration into yeast chromosome III. This is an integrating vector without a yeast replication origin.

Addgene NGS detected a K298R mutation within the HIF1B translation. The depositing laboratory states that this does not adversely affect protein function