pTXRE5-Z
(Plasmid #112509)

• Purpose: Reporter plasmid, responsive to activated Ah receptor/Arnt
• Depositing Lab: Charles Miller
• Publication: Miller CA et al Toxicol Appl Pharmacol. 1999 Nov 1;160(3):297-303. doi: 10.1006/taap.1999.8769.

Backbone

• Vector backbone: unknown
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RW95-3 with 5x xenobiotic response elements
• Alt name: DRE
• Alt name: LacZ
• Species: Synthetic; E.coli
• Entrez Gene: lacZ (a.k.a. b0344, ECK0341)
• Promoter: 5 XREs+ minimal Cytochrome C

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: M13 Reverse

Resource Information

• Supplemental Documents:
  • pTXRE5-Z.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene NGS found that the LacZ translation lacks the last C-terminal 11 amino acids compared to NCBI reference sequence AAB49976.1. The depositing laboratory states that this difference does not adversely affect protein function