pWaldo-GFPe-SH1446
(Plasmid #112507)

• Purpose: Expresses SH1446 in E. coli with a C-terminal TEV-GFP-His(8) tag.
• Depositing Lab: Simon Newstead
• Publication: Minhas et al Elife. 2018 Jul 3;7. pii: 34995. doi: 10.7554/eLife.34995.

Backbone

• Vector backbone: pWALDOe
• Backbone size w/o insert (bp): 5600
• Total vector size (bp): 7000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: for expression use C43 (DE3) bacteria, grow cells at 37°C until induction with IPTG, then grow at 25 °C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: POT family transporter
• Alt name: SH1446
• Species: Staphylococcus hominis
• Insert Size (bp): 1503
• GenBank ID: WP_002449073.1
• Promoter: T7
• Tag / Fusion Protein:
  • C terminal TEV GFP octa-His affinity tag (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (destroyed during cloning)
• 3′ cloning site: bamHI (not destroyed)
• 5′ sequencing primer: AATTAGGAATTCATGGCAACAAATAACTCCCATG
• 3′ sequencing primer: AGGTAGGGATCCGTGAATACCTTTCATAGCTTTCG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry