hCathepsin B
(Plasmid #11249)

• Purpose: Mammalian expression of human cathepsin B
• Depositing Lab: Hyeryun Choe
• Publication: Huang et al J Biol Chem. 2005 Dec 8. ():.

Backbone

• Vector backbone: pcDNA3.1(-)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5428
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cathepsin B
• Species: H. sapiens (human)
• Entrez Gene: CTSB (a.k.a. APPS, CPSB, KWE, RECEUP)
• Tag / Fusion Protein:
  • C9 (GTETSQVAPA) (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: PmeI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

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