pcDNA3.1-hRIPK1-Flag-K377R
(Plasmid
#112489)
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PurposeMammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 112489 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRIPK1
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SpeciesH. sapiens (human)
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MutationK377R
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Entrez GeneRIPK1 (a.k.a. AIEFL, IMD57, RIP, RIP-1, RIP1)
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-hRIPK1-Flag-K377R was a gift from Junying Yuan (Addgene plasmid # 112489 ; http://n2t.net/addgene:112489 ; RRID:Addgene_112489)