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Addgene

MS2-MBP
(Plasmid #11246)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11246 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMAL
  • Backbone manufacturer
    NEB
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MS2-MBP
  • Alt name
    maltose-binding protein
  • Species
    H. sapiens (human)
  • Mutation
    V75Q, A81G in MS2
  • Tag / Fusion Protein
    • MS2 hairpin (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reed lab clone #411.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MS2-MBP was a gift from Robin Reed (Addgene plasmid # 11246)
  • For your References section:

    Purification and electron microscopic visualization of functional human spliceosomes. Zhou Z, Sim J, Griffith J, Reed R. Proc Natl Acad Sci U S A. 2002 Sep 17. 99(19):12203-7. 10.1073/pnas.182427099 PubMed 12215496