Ftz-M3
(Plasmid
#11245)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 11245 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepSP65
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFtz-M3
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Alt nameFtz-three MS2 hairpins
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SpeciesD. melanogaster (fly)
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Tag
/ Fusion Protein
- 3 MS2 hairpins (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Reed lab clone #412.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Ftz-M3 was a gift from Robin Reed (Addgene plasmid # 11245 ; http://n2t.net/addgene:11245 ; RRID:Addgene_11245) -
For your References section:
Purification and electron microscopic visualization of functional human spliceosomes. Zhou Z, Sim J, Griffith J, Reed R. Proc Natl Acad Sci U S A. 2002 Sep 17. 99(19):12203-7. 10.1073/pnas.182427099 PubMed 12215496
Map uploaded by the depositor.
