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Addgene

AdML-M3
(Plasmid #11244)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11244 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSP72
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AdML-M3
  • Alt name
    AdML-three MS2 hairpins
  • Species
    Adenovirus
  • Tag / Fusion Protein
    • 3 MS2 hairpins (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reed lab clone #388.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AdML-M3 was a gift from Robin Reed (Addgene plasmid # 11244 ; http://n2t.net/addgene:11244 ; RRID:Addgene_11244)

  • For your References section:

    Purification and electron microscopic visualization of functional human spliceosomes. Zhou Z, Sim J, Griffith J, Reed R. Proc Natl Acad Sci U S A. 2002 Sep 17. 99(19):12203-7. 10.1073/pnas.182427099 PubMed 12215496
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