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Addgene

SAP145
(Plasmid #11239)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11239 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBS SK+
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SAP145
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2840
  • Entrez Gene
    SF3B2 (a.k.a. CFM, Cus1, HFM, SAP145, SF3B145, SF3b1, SF3b150)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer M13R
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Reed lab clone #130.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SAP145 was a gift from Robin Reed (Addgene plasmid # 11239 ; http://n2t.net/addgene:11239 ; RRID:Addgene_11239)

  • For your References section:

    Evidence that sequence-independent binding of highly conserved U2 snRNP proteins upstream of the branch site is required for assembly of spliceosomal complex A. Gozani O, Feld R, Reed R. Genes Dev. 1996 Jan 15. 10(2):233-43. 10.1101/gad.10.2.233 PubMed 8566756
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