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Addgene

pBS Rad
(Plasmid #11236)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 11236 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Rad
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1300
  • Entrez Gene
    RRAD (a.k.a. RAD, RAD1, REM3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS Rad was a gift from Ronald Kahn (Addgene plasmid # 11236 ; http://n2t.net/addgene:11236 ; RRID:Addgene_11236)

  • For your References section:

    Rad: a member of the Ras family overexpressed in muscle of type II diabetic humans. Reynet C, Kahn CR. Science. 1993 Nov 26. 262(5138):1441-4. 10.1126/science.8248782 PubMed 8248782
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